Anti-Ksp-Cadherin / CDH16(CDH16/1071), CF740 conjugate

Anti-Ksp-Cadherin / CDH16(CDH16/1071), CF740 conjugate

• Description: This MAb recognizes a protein of 130 kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: Cadherin-16 (CDH16); Kidney-specific cadherin; Ksp-cadherin antibody
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CDH16
• Gene ID: 1014
• NCBI Gene ID: 513660
• UniProt: O75309
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Dog, Human, Mouse, Rabbit
• Immunogen: Recombinant human CDH16 protein
• Target Antigen: CDH16 | Ksp-Cadherin
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: CDH16/1071
• Conjugation: CF740
• Disease: Tumor
• Source: Animal
• Applications: IF (verified) | IHC, FFPE (verified) | WB (verified)
• Validated Applications: IF, IHC, FFPE, WB
• Field of Research: Cell adhesion
• Positive Control: Normal kidney or renal cell carcinoma
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% rBSA, 0.05% azide
• Molecular Weight: 130 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years