Anti-BrdU (Bromodeoxyuridine)(85-2C8)

Anti-BrdU (Bromodeoxyuridine)(85-2C8)

• Description: Bromodeoxyuridine (BrdU) is a synthetic nucleoside thymidine analog. It is commonly used to detect proliferation in cells. In cells cultured in the presence of BrdU, BrdU will incorporate into newly synthesized DNA during S-phase. Antibodies specific for BrdU can then be used to detect replicated DNA. This antibody is available purified, with BSA (0.2 mg/mL) or purified, BSA-free (1 mg/mL).
• Synonyms: Bromodeoxyuridine; 5-bromo-2-deoxyuridine
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: Not Applicable
• UniProt: Not Applicable
• Cellular Locus: Nucleus
• Host: Mouse
• Species Reactivity: Pan species
• Immunogen: Bromodeoxyuridine (BrdU) conjugated to hemocyanine (isolated from Helix pomatia)
• Target Antigen: BrdU
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: 85-2C8
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Cancer, Cell cycle
• Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: Depends on the target
• Additionnal Information: Immunofluorescence 0.5-1 ug/mL|Flow Cytometry 0.5-1 ug/million cells/0.1 mL
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years