Anti-bcl-6(BCL6/850), CF568 conjugate

Anti-bcl-6(BCL6/850), CF568 conjugate

• Description: Bcl-6 is a transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. It forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Bcl-6 allows GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. Antibodies to Bcl-6 are helpful in a number of diagnostic settings, for example in the differential diagnosis of small B-cell lymphoma, and in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: Bcl-6; B-Cell Lymphoma 6 Protein; B-cell lymphoma 5 protein; Zinc Finger Protein 51; zinc finger transcription factor BCL6S; BCL6 Transcription Repressor
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: BCL6
• Gene ID: 604
• NCBI Gene ID: 478588
• UniProt: P41182
• Cellular Locus: Nucleus
• Host: Mouse
• Species Reactivity: Human, Mouse
• Immunogen: Recombinant human bcl-6 protein
• Target Antigen: Bcl-6
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: BCL6/850
• Conjugation: CF568
• Disease: Tumor
• Source: Animal
• Field of Research: Apoptosis, Cancer, Immunology, Transcription factor
• Positive Control: Raji or Ramos cells. Tonsil or Hodgkin's lymphoma.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 95 kDa
• Additionnal Information: For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
• References & Citations: Garcia JF, et al. 2006. J. Histochem Cytochem. 54:31. | Pasqualucci, L., et al. 2003. Leuk. Lymphoma 44 Suppl 3: S5-12. | Ree, H.J., et al. 2003. Hum. Pathol. 34: 610-616
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years