Anti-BCL-10(BL10/411)

• Description:

  B-cell lymphoma/leukemia 10 (BCL10) is a signaling protein that promotes apoptosis, pro-caspase-9 maturation, and activation of NF-kappa-B via NIK and IKK. BCL10 contains an N-terminal caspase recruitment domain (CARD), which is found in a number of apoptotic regulatory molecules. BCL10 is involved in the adaptive immune response, and may be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. A BCL10 translocation is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma)Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  BCL10; BCL-10; B-cell CLL/lymphoma 10; B-cell leukemia/lymphoma 10

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  BCL10

• Gene ID:

  8915

• NCBI Gene ID:

  193516

• UniProt:

  O95999

• Cellular Locus:

  Nucleus & cytoplasm

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human BCL10 recombinant protein (epitope aa122-168)

• Target Antigen:

  BCL10

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  BL10/411

• Conjugation:

  Purified, BSA-free

• Source:

  Animal

• Applications:

  Flow, intracellular (verified) | IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  FC, IHC, FFPE, WB

• Field of Research:

  Apoptosis, Cancer, Immunology, Signal transduction

• Positive Control:

  HepG2 cells or Lymphoma.

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  33 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4