Anti-Prostate Specific Antigen (PSA)(KLK3/801 + KLK3/1248)

• Description:

  Recognizes a single protein of 33-34 kDa, identified as the prostate specific antigen (PSA). This MAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

• Synonyms:

  Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  KLK3

• Gene ID:

  354

• NCBI Gene ID:

  171995

• UniProt:

  P07288

• Cellular Locus:

  Secreted (extracellular)

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant full-length human KLK3 protein

• Target Antigen:

  Prostate Specific Antigen

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  KLK3/801 KLK3/1248

• Conjugation:

  Purified, with BSA

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Cancer, Endocrinology

• Positive Control:

  PC12 cells. Normal prostate or prostate carcinoma.

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  33-34 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistochemistry (formalin-fixed): 0.5-1.0 ug/mL for 30 min at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4