Anti-Human Immunoglobulin Gamma (IgG) Heavy Chain(IG217 + IG266)

• Description:

  This MAb is specific to heavy chain of IgG and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of Gamma heavy chain(IgG1, IgG2a, IgG2b, IgG3). The most common feature of plasmacytomas, and certain non-Hodgkin's lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Ab-3 is superb for staining of formalin-fixed tissues.

• Synonyms:

  G1m Marker; G2m Marker; G3m Marker; G4m Marker; HDC; Heavy Chain Disease Protein; Human Immunglobulin G; Ig gamma1/2/3/4 Chain C Region; IGHG1; IGHG2; IGHG3; IGHG4; Immunoglobulin Heavy Constant 1/2/3/4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  IGHG

• Gene ID:

  3500; 3501; 3502; 3503

• NCBI Gene ID:

  510635

• UniProt:

  P01857, P01859, P01860, P01861

• Cellular Locus:

  Secreted (extracellular)

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Purified human IgG

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  IG217 IG266

• Conjugation:

  Purified, BSA-free

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Cancer, Immunology, Inflammation

• Positive Control:

  293T, Raji or hPBL cells. Tonsil or Spleen

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  75 kDa

• Additionnal Information:

  Flow cytometry: 1 ug/million cells|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistochemistry (formalin-fixed): 0.5-1 ug/mL for 30 minutes at RT|Immunofluorescence: 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4