Anti-Fibronectin (8th Type III Repeat)(568), CF640R conjugate

• Description:

  The epitope of this antibody is located in the 8th type III repeat in the cell-binding region of fibronectin. Fibronectins are disulfide-linked, dimeric glycoproteins of ~440 kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb is excellent for staining of formalin-fixed, paraffin-embedded tissues.

• Synonyms:

  Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF); Ugl-Y3

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  FN1

• Gene ID:

  2335

• NCBI Gene ID:

  203717

• UniProt:

  P02751

• Cellular Locus:

  Extracellular matrix

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  High molecular weight proteins secreted by cultivated human fibroblasts

• Target Antigen:

  Fibronectin

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  568

• Conjugation:

  CF640R

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Cell adhesion, Extracellular matrix

• Positive Control:

  SW156 cells. Kidney.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  220 kDa (monomer); 440 kDa (dimer)

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistochemistry (formalin-fixed): 0.5-1 ug/mL for 30 minutes at RT|Flow cytometry: 0.5-1 ug/million cells|Immunofluorescence: 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4