Anti-Cytochrome C (Mitochondrial Marker)(6H2.B4), CF640R conjugate

• Description:

  Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

• Synonyms:

  CYC; CYCS; HCS; THC4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CYCS

• Gene ID:

  54205

• NCBI Gene ID:

  437060

• UniProt:

  P99999

• Cellular Locus:

  Mitochondria

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Rat full-length cytochrome c protein

• Target Antigen:

  Cytochrome c

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  6H2.B4

• Conjugation:

  CF640R

• Source:

  Animal

• Applications:

  Flow, intracellular (verified) | IF (verified)

• Validated Applications:

  FC, IF

• Field of Research:

  Apoptosis

• Positive Control:

  K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  15 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow cytometry: 0.5-1 ug/million cells|Immunofluorescence: 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4