Anti-Cytochrome C (Mitochondrial Marker)(6H2.B4), Biotin conjugate
CAT:
37-BNCB0184-100
Size:
100 µL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Anti-Cytochrome C (Mitochondrial Marker)(6H2.B4), Biotin conjugate
- Description: Cytochrome c is a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.
- Synonyms: CYC; CYCS; HCS; THC4
- CAS Number: 9007-83-4
- UNSPSC: 41116161
- UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
- Gene Name: CYCS
- Gene ID: 54205
- NCBI Gene ID: 437060
- UniProt: P99999
- Cellular Locus: Mitochondria
- Host: Mouse
- Species Reactivity: Human, Mouse, Rat
- Immunogen: Rat full-length cytochrome c protein
- Target Antigen: Cytochrome c
- Clonality: Monoclonal
- Isotype: IgG1 κ
- Clone: 6H2.B4
- Conjugation: Biotin
- Source: Animal
- Applications: Flow, intracellular (verified) | IF (verified)
- Validated Applications: FC, IF
- Field of Research: Apoptosis
- Positive Control: K-562, HL-60, Jurkat, NIH3T3 or PC-3 cells. Liver or Cardiac muscle.
- Concentration: 0.1 mg/mL
- Buffer: PBS, 0.1% BSA, 0.05% azide
- Molecular Weight: 15 kDa
- Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow cytometry: 0.5-1 ug/million cells|Immunofluorescence: 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
- Shipping Conditions: Room temperature
- Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
- Shelf Life: 2 years