Anti-CD36 (Platelet & Microvessel Marker)(1A7), CF640R conjugate

• Description:

  This antibody recognizes a protein of 80-90 kDa, identified as CD36. It is expressed on platelets, monocytes and macrophages, microvascular endothelial cells, erythrocyte precursors, mammary epithelial cells, and some macrophage derived dendritic cells. CD36 acts as a receptor for thrombospondin (TSP), collagen types I, IV and V, P. falciparum malaria-infected erythrocytes, and sickle erythrocytes. It also functions as a scavenger receptor, mediating macrophage uptake of oxidized low-density lipoprotein (LDL) and recognition of apoptotic polymorphonuclear leukocytes (PMN). CD36 plays a role in platelet aggregation, macrophage foam cell development, inflammation, and the tissue ischemia observed in sickle cell disease and cerebral malaria. Note that 1-4% of Japanese and East Asia population lack CD36.

• Synonyms:

  Adipocyte membrane protein; CHDS7; Collagen receptor, platelet; fatty acid translocase (FAT); Fatty acid transport protein; glycoprotein iiib (GP IIIb); PAS IV; PAS-4; pas4 protein; platelet collagen receptor; Platelet glycoprotein 4; scarb3; Scavenger receptor class B member 3; thrombospondin receptor

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CD36

• Gene ID:

  948

• NCBI Gene ID:

  120949

• UniProt:

  P16671

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Human CD36 from platelets

• Target Antigen:

  CD36

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  1A7

• Conjugation:

  CF640R

• Source:

  Animal

• Applications:

  Functional studies (published) | IP (published) | WB (published)

• Validated Applications:

  IP, WB

• Field of Research:

  Immunology, Inflammation

• Positive Control:

  HEL or U937 cells. Platelets, monocytes, macrophages, microvascular endothelial cells in a tonsil.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  80-90 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Flow cytometry: 0.5-1 ug/million cells|Functional Studies Order Ab without Azide|Immunofluorescence: 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Am J Pathol (1999) 155(2): 441-452. (WB) Am J Pathol (2003) 162(3): 771-779. (functional studies, IP)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years