Anti-CD100 (Semaphorin-4D)(A8), Biotin conjugate

• Description:

  CD100 (also known as Semaphorin 4D), is a homodimeric protein comprised of 50 kDa subunits. It is expressed on resting T cells and weakly on other lymphocytes, and its expression is increased on B and T cells after stimulation. CD100 is a cell surface receptor that binds to CD72, Plexin B1 and Plexin B2. It promotes B cell aggregation, cell survival, and cell proliferation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD100; Semaphorin-4D; SEMA4D

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  SEMA4D

• Gene ID:

  10507

• NCBI Gene ID:

  494406

• UniProt:

  Q92854

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human, Monkey, Mouse

• Immunogen:

  PHA stimulated human peripheral blood lymphocytes.

• Target Antigen:

  CD100 | Semaphorin-4D

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  A8

• Conjugation:

  Biotin

• Source:

  Animal

• Applications:

  Flow, surface (published) | ELISA (published) | WB (published)

• Validated Applications:

  FC, ELISA, WB

• Field of Research:

  Immunology, Neuroscience, Signal transduction

• Positive Control:

  Daudi, Raji, HUT-78, Kg1a, U937, and human lymphocytes. Human tonsils and lymph nodes.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  50 kDa (Monomer)

• Additionnal Information:

  Functional studies: Order Ab without azide; Flow cytometry: 0.5-1 ug/million cells; Immunofluorescence: 0.5-1 ug/mL; Optimal dilution for a specific application should be determined.

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. PNAS USA (1996) 93:11780-11785. (Flow) PLoS One (2007) 2(9):e818. (Flow, WB, ELISA)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years