RedDot™1, 200X in water

• Description:

  RedDot trade;1 is a far-red cell membrane-permeable nuclear dye similar to Draq5 trade;. The dye is ideal for specifically staining the nuclei of live cells for short term imaging. RedDot trade;1 can be used to stain adherent or suspension cells. The dye is highly thermostable and photostable, providing convenient handling and ideal for demanding applications such as confocal microscopy. RedDot trade;1 has excitation/emission maxima at 662/694 nm, for detection in the Cy®5 channel. It also can be excited at a wide range of wavelengths between 488 nm and 647 nm, including the 488 nm laser for flow cytometry, and can be used for DNA content analysis by flow cytometry. RedDot trade;1 also can be used for normalization of In Cell Western® in fixed cells, similar to Draq5 trade;/Sapphire®700.

  Please note: far-red dyes like RedDot trade;1 are not visible to the human eye, but must be imaged with a CCD camera or by confocal microscopy.

  Like Draq5®, RedDot trade;1 is toxic to cells within a few hours, and is recommended for short term staining experiments. For a low-toxicity far-red nuclear stain for live cells, see our NucSpot trade; 650 dye. Please also see RedDot trade;2, a spectrally similar dye designed for specific nuclear staining of fixed and permeabilized cells or selective nuclear staining of dead cells.

  Cy dye is a registered trademark of GE Healthcare. Draq5 is a trademark of BioStatus Ltd. In Cell Western and Sapphire are registered trademarks of LI-COR Inc.

• UNSPSC:

  41116113

• UNSPSC Description:

  Cytology reagents or solutions or stains

• Source:

  Synthetic

• Applications:

  Live cell nuclear staining, In Cell Western normalization

• Storage Conditions:

  4°C; Protect from light

• Shelf Life:

  12 months

• Equivalent Products:

  Draq5, CellTag 700, Sapphire 700