Hoechst 33342

• Description:

  Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies and also as nuclear counterstains. Hoechst 33342 is reported to be slightly more membrane-permeable than Hoechst 33258. Either dye can be used to stain live or fixed cells in buffer or medium at 1 ug/mL with no wash step. Biotium also offers Hoechst 33342, 10 mg/mL in H2O.

  • lamba;_Ex/ lamba;_Em (with DNA) = 350/461 nm
  • Pale yellow solid soluble in water up to 2% (w/v)
  • Store at 4°C and protect from light
  • C₂₇H₃₁Cl₃N₆O.3H₂O
  • MW: 615.98
  • [23491-52-3]

• UNSPSC:

  41116113

• UNSPSC Description:

  Cytology reagents or solutions or stains

• Source:

  Synthetic

• Storage Conditions:

  4°C; Store with desiccant; Protect from light

• Shelf Life:

  12 months

• CAS Number:

  23491-52-3