Tyramide Amplification Kit with HRP Streptavidin and CF®680R Tyramide

• Description:

  Tyramide amplification, sometimes called Catalyzed Reporter Deposition (CARD), is an enzyme-mediated detection method that uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. The tyramide amplification method has been reported to increase the sensitivity by up to 100-fold compared to the conventional avidin-biotinylated complex procedure.

  CF® dyes are Biotium's line of next-generation fluorescent dyes, with advantages in brightness and photostability compared to other dyes. Far-red/near-IR fluorescent CF®680R has excitation/emission at 680/701 nm.

  Kit Components:

  • CF®680R tyramide stock solution
  • HRP Streptavidin
  • BSA for blocking
  • Amplification buffer
  • Hydrogen peroxide 30% stablized solution

  We also offer Tyramide Amplification Kits with HRP goat anti-rabbit or HRP streptavidin.

• UNSPSC:

  41116123

• UNSPSC Description:

  Histology test kits or supplies

• Source:

  Animal, Plant; BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells; peroxidase from Horseradish (Armoracia rusticana); recombinant streptadin from E. coli

• Storage Conditions:

  -35°C; Protect from light

• Shelf Life:

  6 months