Human hs-CRP (high sensitivity C Reactive Protein) ELISA Kit

Human hs-CRP (high sensitivity C Reactive Protein) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-CRP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-CRP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-CRP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-CRP in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: C-RP; PTX1; Pentraxin-Related; Pentraxin 1; hs-CRP
• Field of Research: Tumor immunity; Infection immunity; Cardiovascular biology; Rheumatology.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 100 ng/mL
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids
• Detection Range: 1.57-100 ng/mL
• Sensitivity: 0.5 ng/mL