A431 cells stimulated with EGF

A431 cells stimulated with EGF

• Background: A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.
• Synonyms: A431 stimulated with EGF Control Cell lysate
• Conjugation: Unconjugated
• Type: Control Lysate
• Applications: WB
• Field of Research: Ancillary Reagents
• Assay Principle: Sµggested use: A volume of 10-25ul is sµggested for immunobloting.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as solution in LaemmLi electrophoresis sample buffer. Samples need to be boiled for 3-5 minutes, before loading the gel.
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• Shipping Conditions: Room Temperature
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB