Anti-Hu CD294 Alexa Fluor® 488

• Description:

  CD294 (prostaglandin D2 receptor 2) is a G-protein-coupled receptor that is preferentially expressed in CD4+ effector T helper 2 (Th2) cells, but also on eosinophils and basophils. It mediates the pro-inflammatory chemotaxis of eosinophils, basophils, and Th2 lymphocytes generated during allergic inflammation. Single nucleotide polymorphisms in the 3' UTR of CD294 gene have been associated with asthma susceptibility. Outside the immune system CD294 is expressed e.g. in gut, heart, and brain. The intracellular C terminal tail contains sites for phosphorylation by protein kinase C.

• Certification:

  RUO

• Immunogen:

  Human CD294 transfected rat cell line TART/B19-12.10

• Target Antigen:

  CD294

• Clone:

  BM16

• Applications:

  FC (QC tested)

• Format:

  Alexa Fluor® 488

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *De Fanis U, Mori F, Kurnat RJ, Lee WK, Bova M, Adkinson NF, Casolaro V: GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells. GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells. Blood. 2007 May 15;109 (10) :4343-50. Epub 2007 Jan 18., URL: https://pubmed.ncbi.nlm.nih.gov/17234745/, *Mjösberg J, Bernink J, Golebski K, Karrich JJ, Peters CP, Blom B, te Velde AA, Fokkens WJ, van Drunen CM, Spits H: The transcription factor GATA3 is essential for the function of human type 2 innate lymphoid cells. Immunity. 2012 Oct 19;37 (4) :649-59., URL: https://pubmed.ncbi.nlm.nih.gov/23063330/, *Bellinghausen I, Reuter S, Martin H, Maxeiner J, Luxemburger U, Türeci Ö, Grabbe S, Taube C, Saloga J: Enhanced production of CCL18 by tolerogenic dendritic cells is associated with inhibition of allergic airway reactivity. J Allergy Clin Immunol. 2012 Dec;130 (6) :1384-93. , URL: http://www.ncbi.nlm.nih.gov/pubmed/23102918 , *Nagata K, Tanaka K, Ogawa K, Kemmotsu K, Imai T, Yoshie O, Abe H, Tada K, Nakamura M, Sugamura K, Takano S: Selective expression of a novel surface molecule by human Th2 cells in vivo. J Immunol. 1999 Feb 1;162 (3) :1278-86., URL: http://www.ncbi.nlm.nih.gov/pubmed/9973380

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The rat monoclonal antibody BM16 recognizes an extracellular epitope of CD294 / CRTH2 (prostaglandin D2 receptor 2), a G-protein-coupled seven-transmembrane protein expressed on Th2 cells, peripheral blood basophils and eosinophils.

• Applications Notes:

  6

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Rat IgG2a