Biotinylated Human CD59 Protein, His,Avitag™

Biotinylated Human CD59 Protein, His,Avitag™

• Background: CD59, also known as membrane attack complex inhibition factor (MACIF), Protectin, 1F5 antigen, HRF-20 and MIRL. CD59 has been identified as a glycosylphosphatidylinositol-anchored membrane protein that acts as an inhibitor of the formation of the membrane attack complex to regulate complement activation. Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
• Description: Biotinylated Human CD59, His,Avitag (CD9-H82E3) is expressed from human 293 cells (HEK293). It contains AA Leu 26 - Asn 102 (Accession # P13987-1).
• Specifications: This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 12.5 kDa. The protein migrates as 15-20 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Leu 26 - Asn 102
• Host: HEK 293
• Target: CD59
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Human
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Format: Powder
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 12.5 kDa
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C