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Dihydrofolate reductase Rabbit Polyclonal Antibody

CAT: 0013-GTR17691354Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0013-GTR17691354Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Dihydrofolate reductase Rabbit Polyclonal Antibody
Product Name Alternative
Dihydrofolate reductase; 1.5.1.3; DHFR
UniProt
P00374
Reactivity
Human, Mouse, Rat
Cross Reactivity
No cross-reactivity with other proteins
Immunogen
E.coli-derived human DHFR recombinant protein (Position: V2-D187) . Human DHFR shares 90% amino acid (aa) sequence identity with both mouse and rat DHFR.
Target
Dihydrofolate reductase
Clonality
Polyclonal
Conjugation
Unconjugated
Field of Research
Cancer Biology, Cell Biology, Metabolism Research, Protein Biochemistry, Signal Transduction
Purification
Immunogen affinity purified.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Dilution
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
Form
Lyophilized
Molecular Weight
21 kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Tested Applications
FC, ICC, IF, IHC, WB
Host or Source
Rabbit
Preservative
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Isotype
Rabbit IgG

UniProtKB · P00374

Dihydrofolate reductase

DYR_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P00374
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
DHFR
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
1.5.1.3
Processing
—
Secondary accessions
B4DDD2, Q14130, Q6IRW8
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

CytoplasmMitochondrionNucleus

Disease

Disease variant

Biological process

Methotrexate resistanceOne-carbon metabolism

Ligand

NADP

Molecular function

OxidoreductaseRNA-binding

PTM

Ubl conjugation

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