Recombinant Human Granulocyte-macrophage colony-stimulating factor (CSF2) (Active)

Recombinant Human Granulocyte-macrophage colony-stimulating factor (CSF2) (Active)

• CAS Number: 9000-83-3
• Gene Name: CSF2
• UniProt: P04141
• Expression Region: 18-144aa
• Organism: Homo sapiens
• Target Sequence: APARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE
• Tag: N-terminal 6xHis-tagged
• Source: Mammalian cell
• Field of Research: Immunology
• Assay Type: Active Protein & In Stock Protein
• Relevance: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
• Endotoxin: Less than 1.0 EU/ug as determined by LAL method.
• Purity: Greater than 95% as determined by SDS-PAGE.
• Activity: Yes
• Bioactivity: The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 28.1-63.8 pg/mL.
• Length: Full Length of Mature Protein
• Form: Lyophilized powder
• Buffer: Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Molecular Weight: 16.7 kDa
• References & Citations: GM-CSF Primes Proinflammatory Monocyte Responses in Ankylosing Spondylitis. Shi H., Chen L., Ridley A., Zaarour N., Brough I., Caucci C., Smith J.E., Bowness P. Front Immunol 11:1520-1520 (2020)
• Storage Conditions: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
• Protein Length: Full Length of Mature Protein