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Ki-67 antibody

CAT: 0013-GTR18398524-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398524-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Ki-67 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
KI-67; Ki67; KI-67 Antigen (KIA) ; MKI67; Proliferation related Ki-67 antigen
UniProt
P46013
Reactivity
Human
Immunogen
Recombinant human Ki67 protein fragment (around aa 2293-2478) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-267M
Conjugation
Unconjugated
Field of Research
Signal Transduction
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Tonsil (moderate to strong nuclear staining in 80-90% of the germinal centre B-cells and in the vast majority of the suprabasal squamous epithelial cells) . Negative Control: Liver (nuclear staining reaction only in scattered hepatocytes (<1%), cytoplasmic staining is absent or only weak) Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2b, kappa

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