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EpCAM antibody

CAT: 0013-GTR18398503-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398503-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
EpCAM antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TROP1; Tumor-associated Calcium Signal Transducer 1 (TACSTD1) ; ECS-1; Epidermal Surface Antigen 1; ESA1; FLOT2; Flotillin-2; Membrane Component, Chromosome 17, Surface Marker-1 (M17S1) ; REG-1; Reggie-1; Reggie-2
UniProt
P16422
Reactivity
Human
Immunogen
Recombinant fragment from the extracellular domain of human EpCAM protein (around aa100-224) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-326R
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Kidney: In kidney, distal tubule cells must show a strong predominantly membranous staining, while at a least moderate predominantly basolateral staining must be seen in the majority of proximal tubules cells and in scattered epithelial cells lining the Bowman capsule. Negative Control: Tonsil: EpCAM staining should be absent in lymphocytes or smooth muscle cells of the vessels. Cellular Localization: Cell Surface & Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

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