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Annexin V-APC/DAPI Apoptosis Kit

CAT: 0763-E-CK-A258-01Size: 20 AssaysDry Ice: NoHazardous: No
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CAT#:0763-E-CK-A258-01Size:20 Assays
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Detection Method
Fluorometric method; Flow cytometry
Assay Principle
Annexin V is a member of the annexin family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. The Annexin V-labeled fluorescein bind specifically to the PS on the outer leaflet apoptotic cell membrane and can be detected with flow cytometry or fluorescence microscope. Nuclear dyes (PI, DAPI, 7-AAD) have a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. Due to late apoptosis or loss of membrane integrity in necrotic cells, nuclear dyes can enter cells to stain DNA, and when used in combination with Annexin V, cells in different apoptotic stages can be distinguished.
Sample Type
Cell samples
Shipping Conditions
Ice bag
Storage Conditions
This product can be stored at 2-8°C for 12 months with shading light.
Citation 01
https://www.sciencedirect.com/science/article/pii/S1385894724099601
Citation 02
https://pubs.acs.org/doi/abs/10.1021/acsami.4c18570
Citation 03
https://www.sciencedirect.com/science/article/pii/S0304383521005139
Citation 04
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10080018/
Citation 05
https://pubs.rsc.org/en/content/articlelanding/2023/bm/d2bm02140d
Citation 06
https://pubs.rsc.org/en/content/articlelanding/2023/bm/d3bm00813d
Citation 07
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8862181/
Citation 08
https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-023-02640-x
Citation 09
https://cancerandmetabolism.biomedcentral.com/articles/10.1186/s40170-025-00401-6
Citation 10
https://www.cell.com/iscience/fulltext/S2589-0042(25)00895-8
Citation 11
https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fj.202401432R
Citation 12
https://www.sciencedirect.com/science/article/pii/S0898656823003844
Detection Instrument
Flow Cytometer

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