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CD34 antibody

CAT: 0013-GTR18398496-02Size: 0.5 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398496-02Size:0.5 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD34 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
Hematopoietic Progenitor Cell Antigen, HPCA1, Mucosialin
UniProt
P28906
Reactivity
Human
Immunogen
Recombinant full-length human HPCA1 protein
Clonality
Monoclonal
Clone
MSVA-034R
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Liver: A moderate to strong, predominantly membranous staining of endothelial cells should be seen in portal vessels and periportal sinusoidal endothelial cells. Endothelial cells located more remotely from the portal field show a rapidly decreasing CD34 expression level. Negative Control: Liver: Hepatocytes should always stain CD34 negative. Sinusoidal cells remote from the portal fields must also be CD34 negative. Cellular Localization: Cell Surface and Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

UniProtKB · P28906

Hematopoietic progenitor cell antigen CD34

CD34_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P28906
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CD34
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A8K664, Q15970, Q15971, Q5JTA3, Q5JTA4, Q9UJB1
Protein keywords

Coding sequence diversity

Alternative splicing

Biological process

Cell adhesion

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

GlycoproteinPhosphoprotein

Cellular component

Membrane

Domain

SignalTransmembraneTransmembrane helix

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