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CD22 antibody

CAT: 0013-GTR18398488-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398488-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD22 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
B-lymphocyte cell adhesion molecule (BL-CAM) ; B-cell receptor CD22; CD22; Lectin 2; Lyb8; Sialic acid-binding Ig-like lectin 2 (Siglec-2) ; SIGLEC2; T-cell surface antigen Leu-14
UniProt
P20273
Reactivity
Human
Immunogen
Recombinant fragment (around aa52-178) of human BL-CAM/CD22 protein (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-022R
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Tonsil or appendix: A strong, predominantly membranous CD22 staining of the germinal centre and mantle zone B-cells as well as of few interfollicular B-cells should be seen. Negative Control: Tonsil or appendix: A large fraction of the interfollicular lymphocytes and all epithelial cells must not show any CD22 staining. Cellular Localization: Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

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