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CD27 antibody

CAT: 0013-GTR18398487-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398487-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD27 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
LPFS2; S152; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFRSF7; TNFSF7; Tp55; Tumor necrosis factor receptor superfamily member 7
UniProt
P26842
Reactivity
Human
Immunogen
Recombinant human CD27 protein fragment (aa 28-170) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-027M
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation; Cell Biology
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Tonsil: A strong, membranous staining should be seen in the majority of interfollicular lymphocytes but also in a fraction of lymphocytes in the germinal centres and the mantle zone. Negative Control: Tonsil: CD27 staining should be absent in all epithelial cells and a fraction of lymphocytes. Cellular Localization: Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2b, kappa

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