MUC1 / EMA antibody

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CAT#:0013-GTR18398484-01Size:0.1 mL

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Description

MUC1 / EMA antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Name Alternative

Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma- associated mucin Episialin, Epithelial Membrane Antigen (EMA), H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1- alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor-associated epithelial membrane antigen

UniProt

P15941

Reactivity

Human

Immunogen

Recombinant human MUC1 protein

Clonality

Monoclonal

Clone

MSVA-672R

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Dilution

1:75-1:150

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Kidney: Distal tubuli and collecting ducts must show a strong cytoplasmic MUC1 staining while the staining should be at least moderate in the parietal and visceral sheets of the Bowman’s capsule and largely absent in proximal tubuli. Negative Control: Colon: MUC1 staining should be very weak or absent in colonic epithelium. Cellular Localization: Cytoplasmic & Cell Surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Tested Applications

IHC

Host or Source

Rabbit

Isotype

IgG

UniProtKB · P15941

Mucin-1

MUC1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P15941
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: MUC1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: MUC-1
EC number: —
Processing: Precursor
Secondary accessions: A5YRV1, A6ZID9, A6ZIE0, B1AVQ8, B1AVR0, B6ECA1, E7ESE5, E7EUG9, P13931, P15942, P17626, Q0VAP5, Q0VAP6, Q14128, Q14876, Q16437, Q16442, Q16615, Q6S4Y3, Q7Z547, Q7Z548, Q7Z550, Q7Z552, Q9BXA4, Q9UE75, Q9UE76, Q9UQL1, Q9Y4J2

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Autocatalytic cleavageDisulfide bondGlycoproteinLipoproteinPalmitatePhosphoprotein

Cellular component

Cell membraneCytoplasmMembraneNucleusSecreted

Domain

RepeatSignalTransmembraneTransmembrane helix

Disease

Tumor suppressor

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