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S100 antibody

CAT: 0013-GTR18398476-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398476-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
S100 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
NEF; Protein S100-B; S-100 protein beta chain; S100 calcium binding protein beta (neural) ; S100 calcium-binding protein B; S100 protein beta chain; S100B; S100beta
UniProt
P04271
Reactivity
Human
Immunogen
Recombinant full-length human S100B protein
Clonality
Monoclonal
Clone
MSVA-490R
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Appendix: Schwann cells of peripheral nerves and adipocytes should show a strong, predominantly cytoplasmic S100B staining. Negative Control: Appendix: Smooth muscle and epithelial cells must not show any S100B staining. Cellular Localization: Cytoplasmic (and membranes/nuclei) Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

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