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Desmin antibody

CAT: 0013-GTR18398470-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398470-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Desmin antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
CMD1I; CSM1; CSM2; DES; Intermediate filament protein
UniProt
P17661
Reactivity
Human
Immunogen
Recombinant full-length human desmin protein
Clonality
Monoclonal
Clone
MSVA-651M
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Appendix: Smooth muscle cells in the lamina muscularis mucosae and muscularis propria must show a strong cytoplasmic staining while staining should be at least moderate in most smooth muscle cells of blood vessels and in dispersed myofibroblasts. Negative Control: Appendix: Desmin staining should not be seen in lymphocytes and epithelial cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG1, kappa

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