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GATA3 antibody

CAT: 0013-GTR18398460-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398460-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
GATA3 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
GATA3; GATA binding protein-3; GATA-binding factor 3; GATA3; HDR; HDRS; Transacting T-cell-specific transcription factor GATA-3
UniProt
P23771
Reactivity
Human
Immunogen
Recombinant full-length human GATA-3 protein
Clonality
Monoclonal
Clone
MSVA-450M
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
1:50-1:100
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Kidney: A moderate to strong nuclear staining reaction should be seen in a fraction of collecting duct cells and podocytes in glomeruli. ; Tonsil: The vast majority of T helper cells (Th2) in the T-zones should show a weak to moderate nuclear staining. Negative Control: Kidney: Staining should be absent in proximal and distal tubuli as well as in blood vessels. ; Tonsil: Staining should be absent in B-cells. Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:80 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2b, kappa

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