Products for Research Use Only

HER2 / CD340 antibody

CAT: 0013-GTR18398451-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398451-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
HER2 / CD340 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
P185, CD340, Verb b2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog
UniProt
P04626
Reactivity
Human
Immunogen
Recombinant protein encoding the extracellular domain of human c-erbB2
Clonality
Monoclonal
Clone
MSVA-340R
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
1:50-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Colon: Breast cancers with HER2 amplification should show strong positivity. Placenta: A faint staining should be seen at the surface membrane of the syncytiotrophoblast. Negative Control: Breast: Normal epithelial cells must not show HER2 immunostaining. Cellular Localization: Cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG