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PAPP-A antibody

CAT: 0013-GTR18398441-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398441-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PAPP-A antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
ASBABP2; Aspecific BCL2 ARE binding protein 2; Differentially placenta 1 expressed protein; DIPLA1; IGF-dependent; IGFBP4ase; Insulin-like growth factor-dependent IGF-binding protein 4 protease (IGFBP-4 protease) ; PAPA; PAPP A; PAPPA1; Pappalysin-1; Pregnancy Associated Plasma Protein A (PAPP-A)
UniProt
Q13219
Reactivity
Human
Immunogen
Recombinant fragment (within aa 351-523) of human PAPP-A protein (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-780M
Conjugation
Unconjugated
Dilution
1:75-1:150
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Placenta: Trophoplast and chorion cells should show a strong cytoplasmic papp-A staining. Negative Control: Colon: Papp-A immunostaining should be completely absent in both stromal end epithelial cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2b, kappa

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