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MCM7 antibody

CAT: 0013-GTR18398429-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398429-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
MCM7 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
CDC47; DNA replication licensing factor MCM7; MCM7 mini chromosome maintenance deficient 7; Minichromosome Maintenance 7; Mini chromosome maintenance protein 7; P1.1-MCM3; P1CDC47; P85MCM; PNAS146
UniProt
P33993
Reactivity
Human
Immunogen
Recombinant human MCM7 protein fragment (aa195-319)
Clonality
Monoclonal
Clone
MSVA-507R
Conjugation
Unconjugated
Field of Research
Signal Transduction
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Colon: A strong nuclear MCM7 immunostaining should be seen in virtually all crypt base cells. Negative Control: Colon: MCM7 immunostaining should be largely absent in surface epithelial cells and in most stroma cells. Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

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