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Nucleolin antibody

CAT: 0013-GTR18398417-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398417-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Nucleolin antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
NCL; Nucl; Nucleolin; Protein C23
UniProt
P19338
Reactivity
Human
Immunogen
Recombinant full-length human NCL protein
Clonality
Monoclonal
Clone
MSVA-623R
Conjugation
Unconjugated
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Colon: A nuclear nucleolin immunostaining should be seen in all cells. A focus of the staining on nucleoli should be visible – at least – in the superficial epithelial cell layer. Negative Control: Colon: A cytoplasmic or membranous nucleolin immunostaining should be absent in all cells. Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

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