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SOX9 antibody

CAT: 0013-GTR18398414-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398414-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
SOX9 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
Campomelic Dysplasia Autosomal Sex Reversal (CMD1) ; SRA1; SRXX2; SRY (sex determining region Y) box 9; SRY related HMG box gene 9; Transcription factor SOX9
UniProt
P48436
Reactivity
Human
Immunogen
Recombinant humanSOX9 protein fragment (around aa 393- 508) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-709R
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Colon: A strong SOX9 immunostaining should be seen in epithelial cells of the crypt base. Negative Control: Colon: SOX9 immunostaining should be absent in all non-epithelial cell types (except few cells in lymphoid germinal centres – if present) . Cellular Localization: Nucleus Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

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