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Desmoglein 1 antibody

CAT: 0013-GTR18398408-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398408-01Size:0.1 mL
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Description
Desmoglein 1 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
Cadherin family member 4; CDHF4; Desmoglein-1; Desmosomal glycoprotein 1; DG1; DSG1; EPKHE; EPKHIA; Pemphigus foliaceus antigen; PPKS1; SPPK1
UniProt
Q02413
Reactivity
Human
Immunogen
Bovine desmoglein (desmosomal glyco protein I) from nasal epidermis.
Clonality
Monoclonal
Clone
MSVA-544M
Conjugation
Unconjugated
Dilution
1:75-1:150
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Tonsil: A moderate to strong desmoglein 1 immunostaining should be seen in a fraction of squamous epithelial cells. Negative Control: Desmoglein 1 immunostaining should be absent in all non-squamous epithelial cell types. Cellular Localization: Cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2a, kappa

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