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CD23 antibody

CAT: 0013-GTR18398399-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398399-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD23 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
BLAST-2; C-type lectin domain family 4, member J; Fc fragment of IgE low affinity II receptor; Fc-epsilon-RII; FCER2; FCER2A; FceRII; IgE-binding factor (IGEBF) ; Immunoglobulin E receptor, low affinity II; Lymphocyte IgE receptor
UniProt
P06734
Reactivity
Human
Immunogen
Recombinant fragment (around aa 48-321) of human FCER2/CD23 protein (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-023M
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Tonsil: The follicular dendritic cells of the germinal centres should show a strong CD23 immunostaining while the majority of lymphocytes in the mantle zone of the follicles should show an at least weak staining. Negative Control: Tonsil: The squamous epithelial cells and T-cells in the interfollicular T-zones should not show any CD23 immunostaining. Cellular Localization: Cell surface Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
GG1, kappa

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