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PSA

CAT: 0013-GTR18398388-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398388-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
PSA antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
Antigen, prostate-specific (APS), Gamma-seminoprotein, hK3, Kallikrein related peptidase 3, Kallikrein-3 (KLK3), KLK2A1, P-30 antigen, Semenogelase, Seminin
UniProt
P07288
Reactivity
Human
Immunogen
Recombinant fragment (around aa150-250) of human KLK3 protein (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-603R
Conjugation
Unconjugated
Field of Research
Cancer Biology
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Prostate: The epithelial cells of the prostate glands must show a strong cytoplasmic staining. Due to leakage of the antigen in the vicinity of the prostate glands, adjacent stroma cells may display a weak to moderate staining reaction. Negative Control: Colon: PSA immunostaining should be absent in all cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

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