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MTAP antibody

CAT: 0013-GTR18398386-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398386-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
MTAP antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
BDMF; DMSFH; DMSMFH; Epididymis luminal protein 249; HEL249; LGMBF; MeSAdo phosphorylase; Methylthioadenosine phosphorylase; MSAP; MTA phosphorylase; MTAPase; S- methyl-5”-thioadenosine phosphorylase
UniProt
Q13126
Reactivity
Human
Immunogen
Recombinant human MTAP protein fragment (aa95-197) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-741R
Conjugation
Unconjugated
Field of Research
Cancer Biology
Dilution
1:50-1:100
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Ovary: At least a moderate, nuclear and/or cytoplasmic MTAP staining should be seen in ovarian stroma cells. Negative Control: Bladder cancer with homozygous 9p deletion: MTAP staining should be absent in cells from urothelial tumors with homozygous 9p deletion. Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

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