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AIF1 antibody

CAT: 0013-GTR18398407-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398407-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
AIF1 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
Allograft inflammatory factor 1 (AIF1) ; balloon angioplasty responsive transcription (BART1) ; IBA1; Interferon responsive transcript 1; Ionized calcium-binding adapter molecule 1; IRT1; Microglia response factor (MRF1)
UniProt
P55008
Reactivity
Human
Immunogen
Purified recombinant human AIF1 protein (around aa 1-146) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-955M
Conjugation
Unconjugated
Field of Research
Musculoskeletal & Connective Tissue Research
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Colon: An at least moderate to strong cytoplasmic AIF1 immunostaining should be seen in mucosal macrophages. Negative Control: Colon: AIF1 immunostaining should be absent in all non-inflammatory cells. Cellular Localization: Cytoplasmic and cell surface. Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2c, kappa

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