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CD3 antibody

CAT: 0013-GTR18398493-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398493-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD3 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
CD3 epsilon; CD3 TCR complex; T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex
UniProt
P07766
Reactivity
Human
Immunogen
A synthetic peptide from human CD3e protein (around aa 20-40) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-003R
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Tonsil: A moderate to strong membranous staining should be seen in all T-cells in the interfollicular T-zones and in the germinal centres (few cells) . Negative Control: Tonsil: All epithelial structures and B-cells should not show any CD3 immunostaining. Cellular Localization: Cell surface. Cytoplasm Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Rabbit
Isotype
IgG

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