Rabbit anti Rat Sphingosine Kinase 1, Long Form

Rabbit anti Rat Sphingosine Kinase 1, Long Form

• Background: Rat sphingosine kinase 1 catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (spp)(sphingosine + ATP = sphingosine 1-phosphate + ADP), a lipid mediator with both intra- and extracellular functions. also acts on d-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as d,l-threo-dihydrosphingosine, n,n-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The long form of sphingosine kinase 1, an N-terminal extension, is responsible for FceRI-mediated calcium mobilization and may play a role in other ligand mediated signaling events.
• CAS Number: 9007-83-4
• UniProt: NM_133386 or Q1HGK5, Q9NYA1
• Host: Rabbit
• Species Reactivity: Rat
• Isotype: IgG
• Conjugation: Unconjugated
• Type: Polyclonal Antibody
• Applications: WB
• Field of Research: Lipid Mediators
• Purification Method: Ammonium Sulfate Precipitation
• Assay Principle: Antibody suitable for Western blots (1-5 µg/mL). Optimal concentration should be evaluated by serial dilutions.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Venkataraman, K., et al. 'Extracellular export of sphingosine kinase-1a contributes to the vascular S1P gradient.' Biochem. J., 2006, 397, 461-471 2. Ohotski, J., et al. Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer. Br. J. Can. (2012), 106, 1453-1459
• Shipping Conditions: Ambient Temperature, freeze upon arrival
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB