Rabbit anti Mouse Sphingosine Kinase 1 (pS225)

Rabbit anti Mouse Sphingosine Kinase 1 (pS225)

• Background: Activation of Sphingosine kinase (Sphk) increases the bioactive lipid, sphingosine 1-phosphate (S1P) and has been observed in a variety of cancer cells. Phosphorylation of sphingosine by sphingosine kinase (Sphk) is the rate-limiting step in the cellular synthesis of S1P. A principle activation event is the phosphorylation of serine 225 of sphiongosine kinase 1. Inhibition of Sphk1 phosphorylation and hence, Sphk activity is an important target for the development of drugs for cancer, inflammation, cardioprotection as well as a many other indications.
• CAS Number: 9007-83-4
• UniProt: Q8CI15
• Host: Rabbit
• Species Reactivity: Mouse
• Isotype: IgG
• Conjugation: Affinity Purified
• Type: Antigen Immunoaffinity Purified Polyclonal
• Source: Rabbits were immunized with a synthetic peptide incorporating the serine 225 phosphorylation site of mouse sphingosie kinase 1
• Applications: WB, ELISA
• Field of Research: Lipid Mediators
• Purification Method: Antigen Immunoaffiinity Purification
• Assay Principle: Antibody can be used for Western blotting (1-5µg/mL). Antibody shows some cross reactivity (~10% by ELISA) with other phosphoserine containing proteins. Optimal concentration should be evaluated by serial dilutions.
• Stability: See expiration date on vial
• Concentration: Lot Specific See vial for Concentration
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Kihara A, Ikeda M, Kariya Y, Lee EY, Lee YM, Igarashi Y, Sphingosine-1-phosphate lyase is involved in the differentiation of F9 embryonal carcinoma cells to primitive endoderm. J. Biol. Chem. 278 (2003) 14578-14585_x000D_ 2. Choi OH, Kim JH, Kinet JP, Calcium mobilization via sphingosine kinase in signalling by the FceRI antigen receptor. Nature 380 (1996) 634-63_x000D_ 3. Olivera A, Kohama T, Edsall L, Nava V, Cuvillier O, Poulton S, Spiegel S, Sphingosine kinase expression increases intracellular sphingosine-1-phosphate and promotes cell growth and survival. J. Cell Biol. 147 (1999) 545-558_x000D_ 4. Chun J, Goetzl EJ, Hla T, Igarashi Y, Lynch KR, Moolenaar W, Pyne S, Tigyi G, International union of pharacology. XXXIV. Lysophospholipid receptor nomenclature. Pharmacol. Rev. 54 (2002) 265-269_x000D_ 5. Hla T, Signaling and biological actions of sphingosine 1-phosphate. Pharmacol. Res. 47 (2003) 401-407_x000D_ 6. Gregorius K, Mouritsen S, Elsner HI, Hydrocoating: a new method for coupling biomolecules to solid phases. J. Immunol. Methods, 181 (1995) 65-73_x000D_ 7. Zhang JH, Chung TDY, Oldenburg KR, A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screening, 4 (1999) 67-73_x000D_ 8. Ohotski, J., et al. Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer. Br. J. Can. (2012), 106, 1453-1459
• Shipping Conditions: Ambient Temperature, freeze upon arrival
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB