Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)

Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)

• Background: T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
• Description: Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (CDG-C5257) is expressed from human 293 cells (HEK293). It contains AA Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G) (Accession # Q95LI5-1 (CD3E) & Q95LI7-1 (CD3G)).
• Specifications: Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of CD3E and CD3G, has a calculated MW of 40.6 kDa (CD3E) and 40.4 kDa (CD3G). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus and subunit CD3G is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) heterodimer protein migrates as 45-52 kDa due to glycosylation.
• Expression Region: Gln 22 - Asp 117 (CD3E) & Gln 23 - Thr 113 (CD3G)
• Host: HEK 293
• Target: CD3E & CD3G
• Conjugation: Unconjugated
• Tag: C-Fc | C-Fc
• Source: Cynomolgus
• Stability: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution._x000D_For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Endotoxin: 1.0 EU per μg
• Purity: 95%
• Bioactivity: Immobilized Bispecific T cell Engager (CD3 X BCMA) at 1 μg/mL (100 μL/well) can bind Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag(Cat. No. CDG-C5257) with a linear range of 0.3-5 ng/mL (QC tested).
• Format: Powder
• Buffer: Tris with Glycine, Arginine and NaCl, pH7.5
• Additives: Trehalose
• Molecular Weight: 40.6 kDa (CD3E) & 40.4 kDa (CD3G)
• Shipping Conditions: Room Temperature
• Storage Conditions: -20°C