Biotinylated Human HLA-A*24:02&B2M&WT-1 (CYTWNQMNL) Complex Protein (Monomer, MALS verified)

Biotinylated Human HLA-A*24:02&B2M&WT-1 (CYTWNQMNL) Complex Protein (Monomer, MALS verified)

• Background: Wilms tumor gene 1 (WT1)，is an attractive target antigen for leukemia and solid cancer. WT1-specific adoptive immunotherapy has developed for tumor treatment in recent years. WT1 has been proved wildly expressed in breast, colon and ovarian cancer. It participates in cell growth, differentiation and apoptosis regulation. The human HLA-A*2402 WT-1 (CYTWNQMNL) complex protein is a complex of HLA-A*2402 of the MHC Class I, B2M and CYTWNQMNL peptide of the WT-1.
• Description: Biotinylated Human HLA-A*24:02&B2M&WT-1 (CYTWNQMNL) Complex Protein (HLW-H82E6) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Thr 305 (HLA-A*24:02) & Ile 21 - Met 119 (B2M) & CYTWNQMNL peptide (Accession # AAA59600.1 (HLA-A*24:02) & P61769 (B2M) & CYTWNQMNL).
• Specifications: Biotinylated Human HLA-A*24:02&B2M&WT-1 (CYTWNQMNL) Complex Protein is produced by co-expression of HLA and B2M loaded with WT-1 peptide. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 35.8 kDa and 11.7 kDa. The protein migrates as 39-41 kDa and 10 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expression Region: Gly 25 - Thr 305 (HLA-A*24:02) & Ile 21 - Met 119 (B2M) & CYTWNQMNL peptide
• Host: HEK 293
• Target: HLA-A*2402 & B2M & WT-1 (CYTWNQMNL)
• Conjugation: Biotin-labeled
• Tag: C-10×His & C-Avi
• Source: Human
• Stability: The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.
• Endotoxin: 1.0 EU per μg
• Purity: 90%
• Bioactivity: Immobilized Biotinylated Human HLA-A*24:02&B2M&WT-1 (CYTWNQMNL) Complex Protein (Cat. No. HLW-H82E6) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-1 ng/mL (QC tested).
• Format: Liquid
• Buffer: PBS, pH7.4
• Additives: Trehalose
• Molecular Weight: 35.8 kDa and 11.7 kDa
• Shipping Conditions: Dry ice
• Storage Conditions: -70°C