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Human FGF 21 Protein

CAT: 0749-PRP1030-01Size: 5 µgDry Ice: NoHazardous: No
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CAT#:0749-PRP1030-01Size:5 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Fibroblast Growth Factor 21 (FGF21) is a growth factor that belongs to the FGF family. Proteins of this family possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGFs act through a family of four FGF receptors. Binding is complicated and requires both interaction of the FGF molecule with an FGF receptor and binding to heparin through an heparin binding domain. Endocrine FGFs lack a heparin binding domain and thus can be released into the circulation. FGF21 action through one of the FGF21 receptors thus requires interaction with a co-receptor, designated β-klotho.
Description
Human FGF21 protein, expressed in E. coli
Product Name Alternative
Fibroblast Growth Factor 21; FGF-21; FGF21
Gene ID
26291
Accession Number
Q9NSA1
Tag
FGF21
Type
Cytokines and proteins
Applications
No tag
Purity
≥ 98 % as determined by SDS-PAGE
Activity
Testing in progress
Molecular Weight
19.4 kDa
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Endotoxin: < 1 EU per μg of the protein as determined by the LAL method
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Lyophilized Human FGF21 proteinshould be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.
Protein Length
The recombinant Human FGF21 consists of 181 amino acids and has a predicted molecular mass of 19.4 kDa. The apparent molecular mass of the Human FGF21 protein is approximately 19.4 kDa in SDS-PAGE under reducing conditions
Formulation
Lyophilized from sterile 20 mM Tris, 150 mM NaCl pH 8.0.
Applications Notes
Human
Other Product Names
Fibroblast Growth Factor 21; FGF-21; FGF21
Recommended Usage
Always centrifuge tubes before opening . It is recommended to centrifuge at 12000 rpm for 1 minute.Then reconstitute the lyophilized Human FGF21 protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Preparation
E. coli
Accession Number URL
http://www.uniprot.org/uniprot/ Q9NSA1
AA Sequence
Amino acid sequence derived from Human FGF21 isoform (Q9NSA1) (His29-Ser209) was expressed.

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