Restriction enzyme FokI (C-term) Rabbit Polyclonal Antibody

Restriction enzyme FokI (C-term) Rabbit Polyclonal Antibody

• Description: The enzyme FokI, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3':3'-CATCC-5' recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site. Its molecular mass is 65.4 kDa, being composed of 587 amino acids.
• Abbreviation: R.FokI
• CAS Number: 9007-83-4
• Swiss Prot: P14870 Planomicrobium okeanokoites
• Host: Rabbit
• Species Reactivity: Planomicrobium okeanokoites
• Immunogen: Synthetic peptide within C-terminal of Planomicrobium okeanokoites fokIR
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Rabbit polyclonal Antibody
• Applications: WB
• Positive Control: Purified restriction enzyme FokI
• Concentration: 1 mg/mL
• Dilution: WB: 1:1,000-1:2,000
• Purity: Immunogen affinity purified.
• Form: Liquid
• Buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 66 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: No