Restriction enzyme FokI (C-term) Rabbit Polyclonal Antibody
CAT:
992-R1501-3
Size:
100 µL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Restriction enzyme FokI (C-term) Rabbit Polyclonal Antibody
- Description: The enzyme FokI, naturally found in Flavobacterium okeanokoites, is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal. Once the protein is bound to duplex DNA via its DNA-binding domain at the 5'-GGATG-3':3'-CATCC-5' recognition site, the DNA cleavage domain is activated and cleaves, without further sequence specificity, the first strand 9 nucleotides downstream and the second strand 13 nucleotides upstream of the nearest nucleotide of the recognition site. Its molecular mass is 65.4 kDa, being composed of 587 amino acids.
- Abbreviation: R.FokI
- CAS Number: 9007-83-4
- Swiss Prot: P14870 Planomicrobium okeanokoites
- Host: Rabbit
- Species Reactivity: Planomicrobium okeanokoites
- Immunogen: Synthetic peptide within C-terminal of Planomicrobium okeanokoites fokIR
- Isotype: IgG
- Conjugation: Non-conjugated
- Type: Rabbit polyclonal Antibody
- Applications: WB
- Positive Control: Purified restriction enzyme FokI
- Concentration: 1 mg/mL
- Dilution: WB: 1:1,000-1:2,000
- Purity: Immunogen affinity purified.
- Form: Liquid
- Buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- Molecular Weight: Predicted band size: 66 kDa
- Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Recombinant Antibody: No