PD-L1 Recombinant Rabbit Monoclonal Antibody [PD01-02]

PD-L1 Recombinant Rabbit Monoclonal Antibody [PD01-02]

• Description: PD-L1 (programmed-death ligand 1; CD274), is a transmembrane protein constitutionally expressed on a variety of cell types, including antigen presenting cells (dendritic cells and histiocytes) and some non-lymphoid tissues (heart and lung). Binding of PD-L1 to PD-1 (programmed-death 1; CD279) expressed by activated T-cells, inhibits their function, causing negative feedback control of immunological reactions, thus impeding inflammation and autoimmunity. Tumour cells may express PD-L1, which binds to PD-1 allowing cancer cells to evade the attack of T-cells. Blockade of the PD-1/PD-L1 pathway has now shown useful in therapy of multiple cancer types, causing durable tumour regressions in a substantial proportion of otherwise treatment refractory cases of melanoma, and carcinomas of e.g., lung, kidney, and urinary tract. Patients without tumour PD-L1 expression can also derive benefit from blocking agents (studies across multiple cancer types demonstrate a pooled response rate of 48% in patients with PD-L1-positive tumours compared to 15% in PD-L1-negative tumours). Tonsil and placenta can be used as positive and negative tissue controls. However, tonsil is found to be superior to placenta, as tonsil displayes a range of PD-L1 expression levels. Tonsil displayes the following reaction pattern: No staining reaction in the vast majority of lymphocytes including mantle zone and germinal centre B-cells, no staining reaction in superficial epithelial cells, a weak to moderate, typically punctuated membranous staining reaction of the majority of germinal centre macrophages and finally a moderate to strong staining reaction of the majority of epithelial crypt cells.
• Product Name Alternative: B7 H antibody B7 H1 antibody B7 homolog 1 antibody B7-H1 antibody B7H antibody B7H1 antibody CD 274 antibody CD-274 antibody CD274 antibody CD274 antigen antibody CD274 molecule antibody MGC142294 antibody MGC142296 antibody OTTHUMP00000021029 antibody PD L1 antibody PD-L1 antibody PD1L1_HUMAN antibody PDCD1 ligand 1 antibody PDCD1L1 antibody PDCD1LG1 antibody PDL 1 antibody PDL1 antibody Programmed cell death 1 ligand 1 antibody Programmed death ligand 1 antibody RGD1566211 antibody
• Abbreviation: CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1
• CAS Number: 9000-83-3
• Swiss Prot: Q9NZQ7 Human
• Cellular Locus: Cell membrane, Early endosome membrane, Recycling endosome membrane.
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Synthetic peptide within human PD-L1 aa 260-290 (Cytoplasmic).
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: IHC-P, mIHC, WB
• Positive Control: Human Small Cell Lung Cancer, human non-small cell lung cancer, MDA-MB-231 cell lysate, U-87 MG cell lysate, HeLa cell lysate, A549 treated with 100ng/mL IFN gamma for 48 hours cell lysate, human lung carcinoma tissue, human placenta tissue.
• Concentration: 0.977 mg/mL
• Dilution: IHC-P: 1:200-1:800 ;mIHC: 1:1,000 ;WB: 1:2,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 33 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes