MDH2 Recombinant Rabbit Monoclonal Antibody [JE64-68]

• Description:

  Malate dehydrogenase, mitochondrial also known as malate dehydrogenase 2 is an enzyme that in humans is encoded by the MDH2 gene. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Mutations in the MDH2 gene have been associated with several cancers, including uterine cancer, prostate cancer, pheochromocytoma and other paragangliomas. In particular, MDH2 has been found to be overexpressed in doxorubicin-resistant uterine cancer cells and may contribute to drug resistance. Since MDH2 plays a major role in malate-aspartate shuttling in ATP production, its overexpression likely supplies additional energy for P-glycoprotein to pump chemotherapeutic drugs out of the cells. Likewise, MDH2 contributes to docetaxel resistance in prostate cancer cells via the JNK pathway, and its knockdown reduced ATP levels as well as increased drug sensitivity. Thus, MDH2 may be an effective therapeutic target to enhance drug treatments for cancer.

• Product Name Alternative:

  M MDH antibody Malate dehydrogenase 2, NAD (mitochondrial) antibody Malate dehydrogenase antibody Malate dehydrogenase, mitochondrial antibody MDH antibody mdh2 antibody MDHM_HUMAN antibody MGC:3559 antibody mitochondrial antibody Mitochondrial malate dehydrogenase 2, NAD antibody Mor 1 antibody MOR1 antibody

• Abbreviation:

  MDH2

• Swiss Prot:

  P40926 Human, P08249 Mouse, P04636 Rat

• Cellular Locus:

  Mitochondrion matrix.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Synthetic peptide within human MDH2 aa 101-150/338.

• Isotype:

  IgG

• Conjugation:

  Non-conjugated

• Type:

  Recombinant Rabbit monoclonal Antibody

• Applications:

  WB, IHC-P

• Positive Control:

  Rat lung tissue lysate, Hela cell lysate, rat brain tissue lysate, mouse kidney tissue lysate, rat testis tissue, human colon carcinoma tissue, human breast carcinoma tissue, mouse hippocampus tissue, mouse brain tissue.

• Concentration:

  1 mg/mL

• Dilution:

  WB: 1:500-1:1,000 ;IHC-P: 1:200-1:400

• Purity:

  Protein A affinity purified.

• Form:

  Liquid

• Buffer:

  1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Molecular Weight:

  Predicted band size: 36 kDa

• Storage Conditions:

  Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.

• Recombinant Antibody:

  Yes

• CAS Number:

  9000-83-3