Actin Recombinant Rabbit Monoclonal Antibody [JF47-01]

Actin Recombinant Rabbit Monoclonal Antibody [JF47-01]

• Description: All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. α-Actin expression is limited to various types of muscle, whereas β-Actin and γ-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.
• Product Name Alternative: a actin antibody ACTA antibody ACTA1 antibody Actin alpha skeletal muscle antibody Actin antibody actin, alpha 1, skeletal muscle 1 antibody actin, alpha 1, skeletal muscle antibody Actin, alpha skeletal muscle antibody actina antibody actine antibody ACTS_HUMAN antibody aktin antibody Alpha Actin 1 antibody Alpha skeletal muscle Actin antibody alpha skeletal muscle antibody alpha-actin antibody Alpha-actin-1 antibody ASMA antibody CFTD antibody CFTD1 antibody CFTDM antibody MPFD antibody NEM1 antibody NEM2 antibody NEM3 antibody nemaline myopathy type 3 antibody
• Abbreviation: ACTA1
• CAS Number: 9000-83-3
• Swiss Prot: P68133 Human, P68134 Mouse, P68136 Rat
• Cellular Locus: Cytoplasm.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat, Zebrafish
• Immunogen: Synthetic peptide within Human Actin aa 303-349 / 377.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IF-Cell, FC
• Positive Control: HeLa cell lysate, C2C12 cell lysate, L6 cell lysate, PC-12 cell lysate, COS-1 cell lysate, Zebrafish tissue lysate, Hybrid fish (crucian-carp) brain tissue lysate, Hybrid fish (crucian-carp) kidney tissue lysate, human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, HeLa, NIH/3T3, L6, PC-12.
• Concentration: 1 mg/mL
• Dilution: WB: 1:5,000-1:20,000 ;IHC-P: 1:200 ;IF-Cell: 1:250-1:500 ;FC: 1:100-1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 42 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Recombinant Antibody: Yes